环状RNA芯片-康成生物丨数谱生物
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      1. 环状RNA芯片

        • 简介
        • 芯片特点
        • 实验流程
        • 客户案例
        • SCI成果
        •        环状RNA(circRNA)是一类由特殊剪接机制形成的、具有闭合环状结构、大量存在于真核转录组中的非编码RNA;也是目前生命科学和医学领域的研究热点分子。circRNA分子的组织特异性、疾病特异性、时序特异性及高稳定性等特征,使得circRNA作为临床疾病的biomarker具有明显的优势。近来研究显示,环状RNA在不同物种中起到miRNA海绵的作用,称之为竞争性内源RNA(ceRNA),即能竞争性结合miRNA。而与疾病关联性miRNA的相互作用说明环状RNA对疾病的调控起着非常重要的作用。此外,一些内含子类型的环状RNA(ciRNA)会促进宿主基因的转录。
                 为了能够更好地研究circRNA,美国Arraystar时时彩官方开奖在全球首款circRNA芯片基础上迅即升级版本为V2.0。其circRNA来源融合了环状RNA研究的最新顶尖文献,所有cicrRNA都经过了严谨的实验验证,以便于对不同生理及病理条件下的circRNA进行系统的研究。同时我们对所有差异表达的circRNA用高匹配值的miRNA靶标位点进行了标注,这将有利于对circRNA作为天然miRNA海绵功能的进行研究。

          Aksomics(康成生物)是Arraystar时时彩官方开奖中国地区唯一代理商,为您提供一站式circRNA芯片技术服务,您只需要提供保存完好的组织或细胞标本,Aksomics的技术服务人员就可为您完成全部实验操作,并提供完整的实验报告。

          Arraystar时时彩官方开奖circRNA芯片时时彩官方开奖中奖列表


          服务 芯片 规格 描述
          Human circular RNA Microarray Service Human circular RNA Array V2.0 8x15K 13,617 human circular RNAs
          Mouse circular RNA Microarray Service Mouse circular RNA Array V2.0 8×15K 14,236 Mouse circular RNAs
          Rat circular RNA Microarray Service Rat circular RNA Array V2.0 8×15K 14,145 Rat circular RNAs
        • ● 全面、系统的circRNA收集:circRNA研究领域顶尖期刊和权威公共数据库,运用强大的信息筛选系统,挑选出最可靠的circRNA。

          ● 高效、精准的circRNA检测:剪接位点特异性探针与Rnase R预处理双重保障,即便相应线性RNA存在也能特异性检测circRNA。

          ● 严谨、可靠的Spike-in对照:在RNA样品中加入一组由ERCC (External RNA Controls Consortium)开发的外源性RNA,可对RNA扩增,标记和芯片杂交过程中产生的实验偏差进行校正,增加样本间结果比较的可靠性。

          ● 详尽、丰富的circRNA注释:Arraystar miRNA靶基因预测软件对circRNA上的保守miRNA的结合位点进行了预测,并对其中匹配分值较高的位点进行了注释(下图)。此外,我们还提供了与circRNA对应的线性mRNA信息。根据信息,客户可以很方便地从不同角度研究circRNA的作用机制和生物功能。


        • 1. 样品RNA抽提
                 实验对象为组织样品,取适量(50-100mg)新鲜组织样品或正确保存的组织样品,加1ml的RNA抽提试剂TRIzol(Invitrogen),匀浆后抽提RNA。
                 实验对象为细胞样品,每份样品取1×106~1×107细胞,完全吸去培养液后加1ml的RNA抽提试剂TRIzol,裂解后抽提RNA。
          2.  RNA质量检测
                 使用Nanodrop测定RNA在分光光度计260nm、280nm和230nm的吸收值,以计算浓度并评估纯度。
                 用甲醛电泳试剂进行变性琼脂糖凝胶电泳,检测RNA纯度及完整性。
                 提供RNA QC报告。
          3. RNase R处理
          4. cDNA/aRNA样品合成和标记
          5. 标记效率质量检测
                 使用Nanodrop检测荧光标记效率,标记效率合格以保证后续芯片实验结果的可靠性。
          6. 芯片杂交
                 在标准条件下将标记好的探针和高密度基因组芯片进行杂交。
          7. 图像采集和数据分析
                 使用GenePix 4000B芯片扫描仪扫描芯片的荧光强度,并将实验结果转换成数字型数据保存,使用配套软件对原始数据进行分析运算。
          8. 提供实验报告:
                 • Scanning Image: Cy3荧光扫描图像;
                 • Scatter Plot:散点图,X轴为Control信号值,Y轴为Experiment信号值,表示信号数据总体分布趋势;
                 • Raw Data:探针的扫描荧光信号强度原始数据;
                 • Normalized Data:经过统计学方法标准化后数值;
                 • P–value: T-test统计分析显著差异表达的基因,p-value越小,该circRNA在两组样本间差异表达越显著;        

                 • Significant Up & Down Regulated CircRNA List:显著差异表达的circRNA列表包含Fold Change, p-value。


        • circRNA在免疫衰老中的相关研究(Comprehensive circular RNA profiling reveals that circular RNA100783 is involved in chronic CD28-associated CD8(+)T cell ageing. Immunity & Ageing, 2015)

                 生物机体免疫系统随着年龄的增长而不断恶化,这个过程被称为免疫衰老(immunosenescence);免疫衰老与系统炎症反应、慢性炎症疾病及癌症发生等密切相关,但目前对引发免疫衰老的机制了解的甚少。该研究通过Arraystar circRNA芯片筛选发现:在健康成人与免疫力低下的老年人的外周血淋巴细胞中,circRNA的表达谱具有明显的差异。在差异表达的circRNA中,研究者通过qPCR验证发现hsa_circRNA_100783可以明显区分两种不同类型的样本,可以作为免疫衰老的biomarker。研究者接着对circRNA可能结合的miRNA进行了靶基因预测,推测circRNA很可能通过调控磷酸蛋白信号转导途径来影响免疫细胞衰老的过程。

        • → A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion. Immunity. 2018

          → Circular RNA MTO1 acts as the sponge of miR-9 to suppress hepatocellular carcinoma progression.  Hepatology (Baltimore, Md.). 2017

          → circRNA Mediates Silica-Induced Macrophage Activation Via HECTD1/ZC3H12A-Dependent Ubiquitination. Theranostics. 2017

          → A Circular RNA Binds To and Activates AKT Phosphorylation and Nuclear Localization Reducing Apoptosis and Enhancing Cardiac Repair.Theranostics. 2017

          → The circular RNA circPRKCI promotes tumor growth in lung adenocarcinoma. Cancer Research. 2018

          → circRNA_100290 plays a role in oral cancer by functioning as a sponge of the miR-29 family. Oncogene. 2017

          → Silencing circular RNA hsa_circ_0000977 suppresses pancreatic ductal adenocarcinoma progression by stimulating miR-874-3p and inhibiting PLK1 expression. Cancer Letters. 2018

          → Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression. Molecular Cancer. 2017

          → Circular RNA expression is suppressed by androgen receptor (AR)-regulated adenosine deaminase that acts on RNA (ADAR1) in human hepatocellular carcinoma. Cell Death & Disease. 2017

          → Comprehensive Circular RNA Profiling Reveals That hsa_circ_0005075, a New Circular RNA Biomarker, Is Involved in Hepatocellular Crcinoma Development. Medicine. 2016

          → CircRNA_000203 enhances the expression of fibrosis-associated genes by derepressing targets of miR-26b-5p, Col1a2 and CTGF, in cardiac fibroblasts. Scientific Reports. 2017

          → Circular RNA Related to the Chondrocyte ECM Regulates MMP13 Expression by Functioning as a MiR-136 'Sponge' in Human Cartilage Degradation. Scientific Reports. 2016

          → Circular RNA GRB10 as a competitive endogenous RNA regulating nucleus pulposus cells death in degenerative intervertebral disk. Cell Death & Disease. 2018

          → circGFRA1 and GFRA1 act as ceRNAs in triple negative breast cancer by regulating miR-34a. Journal of Experimental & Clinical Cancer Research. 2017

          → Circ100284, via miR-217 regulation of EZH2, is involved in the arsenite-accelerated cell cycle of human keratinocytes in carcinogenesis. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2017

          → CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients. Cellular Physiology and Biochemistry. 2017

          → Altered Circular RNA Expression in Patients with Repeated Implantation Failure. Cellular Physiology and Biochemistry. 2017

          → Microarray Expression Profile and Functional Analysis of Circular RNAs in Osteosarcoma. Cellular Physiology and Biochemistry. 2017

          → Microarray Expression Profile of Circular RNAs in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis Patients.Cellular Physiology and Biochemistry. 2017

          → Circular RNA circ-4099 is induced by TNF-α and regulates ECM synthesis by blocking miR-616-5p inhibition of Sox9 in intervertebral disc degeneration. Experimental & molecular medicine. 2018




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